梅花鹿体内脑肠肽的表达及细胞定位分析

doi:10.11843/j.issn.0366-6964.2017.01.019

Abstract

Abstract:

In order to specify the Ghrelin expression and localization inside sika deer, we adopted the reverse transcriptase-polymerase chain reaction (RT-PCR), real-time fluorescent quantitative PCR (RT-qPCR ) and immunohistochemical technique to test the relative expression quantity of Ghrelin mRNA and Ghrelin protein location distribution in the esophagus, rumen, reticulum, omasum, abomasum, duodenum, jejunum, ileum, caecum, colon, liver, pancreas, thyroid, anterior pituitary, lung, kidney, spleen, ovarian and other organs of the sika deer. RT-qPCR results showed: Ghrelin mRNA was expressed in these organs, and the expression quantity in the abomasum was significantly higher than that of other organs (P<0.05); Immunohistochemical staining result showed that Ghrelin protein was expressed in these organs, most widely distributed in the digestive tract. The specific expression pattern of Ghrelin mRNA and Ghrelin protein inside Sika deer revealed that the new molecule may have extensive biological effect in the growth and development process of sika deer, and existed as an important adjusting mode especially in the aspect of gastrointestinal function.

CLC Number:

S852.1

LIU Jiao, ZHANG Man, JIN Xin, FAN Yan-ru, TIAN Qiao-zhen, YANG Yin-feng. Ghrelin Expression and Cell Location Analysis inside Sika Deer[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(1): 157-165.

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Ghrelin Expression and Cell Location Analysis inside Sika Deer

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